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The PA 800 plus Carbohydrate Labeling and Analysis Chemistries contain the reagents, buffers and separation capillaries required to label, separate and quantify oligosaccharides and monosaccharides released from glycoproteins. After release (enzymatic or chemical), sugars are labeled with a fluorophore, APTS, at the reducing termini by reductive amination. The stoichiometry of labeling is such that only one APTS molecule is attached to each molecule of oligosaccharide. These highly charged and fluorescent oligosaccharides are easily resolved in an electric field and detected by laser-induced fluorescence.


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Carbohydrate Labeling and Analysis Chemistries Include: 
  ▪ Carbohydrate Separation Buffer, 56 mL
  ▪ N-CHO Coated Capillary, 2 pieces
  ▪ Labeling Reagents, 1 piece
  ▪ Labeling Dye (APTS), 4 x 5 mg
  ▪ Labeling Dye Solvent, 1 mL
  ▪ Glucose Ladder Standard, 50 mg
  ▪ Quantitation/Mobility Marker (Maltose), 0.18 mg
  ▪ APTS-M (monosaccharides grade), 20 mg
  ▪ Carbohydrate Labeling and Analysis Guide, 1 piece
 
System and Chemistry Features:
  ▪ Unique capillary cooling
  ▪ Unique high resolution separation methods
  ▪ Ability to separate positional isomers of glycoproteins
  ▪ Unique sample cooling
  ▪ Quantitative
  ▪ Automated
  ▪ Optimized methodology

System Part Number

Click on the Part Number(s) below for Ordering Information.

Part Number
Description
477600 Carbohydrate Labeling and Analysis Chemistry

“CE Separation of N-Linked Oligosaccharides Released from Recombinant Monoclonal Antibody” (AIB A-1986A) - This bulletin describes the isolation, separation and profiling analysis of complex N-linked oligosaccharides by CE using the Carbohydrate Labeling and Analysis Assay.

Click here for the Carbohydrate Labeling and Analysis Kit Insert.

Click here for more Information on the Carbohydrate Labeling & Analysis Application.