Agarose slab gel electrophoresis has long been the typical method used for the analysis of DNA and RNA. Although well established, it is time consuming, labor intensive and only semi-quantitative at best. Capillary gel electrophoresis offers an automated approach to nucleic acid analysis with on-line detection that is fully quantitative, extending the analytical capabilities of electrophoresis. Gels exist as polymers in solution, allowing the user an environment which can easily be optimized. Polymers in solution also allow for an automated mechanism of gel loading and gel replacement. Beckman Coulter offers the following nucleic acid kits useful for the routine analysis of oligonucleotides, viral load determination, gene expression and the quantitative analysis of DNA-protein interactions through mobility-shift assays.
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| eCAP™ dsDNA 20,000 Kit, Part Number 477486 |
| The eCAP™ dsDNA 20,000 Kit was designed for the analysis of double-stranded DNA fragments from 1,000 to 20,000 base pairs but fragments smaller than 1,000 base pairs will be detected. The eCAP™ dsDNA 20,000 Kit includes two coated capillaries, three vials of replaceable gel buffer, test mix, and instructions.
This kit is compatible with both UV and LIF detection. It is not compatible with PDA detection. For LIF applications, eCAP™ EnhanCE intercalating dye is recommended.
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Before and after separations of DNA fragments derived from the complete EcoR1 digestion of a 12 Kbp plasmid vs. the undigested plasma itself. |
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Part Number |
Description |
| 477477 |
eCAP™ DNA Capillary - 100 µm ID, 65 cm total length (quantity 1) |
| 477485 |
eCAP™ dsDNA 20,000 Gel Buffer (pack of 3) |
| 477483 |
eCAP™ Orange G Reference Marker |
| 241524 |
eCAP™ Orange G Reference Marker |
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| eCAP™ dsDNA 1000 Kit, Part Number 477410 |
| For DNA fragment pattern recognition, quantitation of PCR fragments, and molecular sizing determinations. Typical resolution for fragments less than 400 base pairs is 5-15. Molecular size linearity from 100 to 1,000 base pairs. The kit includes a coated capillary and replaceable gel, test mix, and an internal standard to perform at least 100 runs.
This kit is compatible with both UV and LIF detection. It is not compatible with PDA detection. For LIF applications, eCAP™ EnhanCE intercalating dye is recommended.
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Separation of an Hae III Restriction
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Separation of an Hae III restriction digest of fX174 DNA using LIF detection. Pressure injection for 10 seconds (10 µg/mL sample). |
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CE-LIF analysis of DNA products
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CE-LIF analysis of DNA products generated by competitive RNA-PCR* of cytochrome P450-1A1 mRNA in dioxin-treated HepG2 cells. Target sequence is 367 bp; competitor is 228 bp; CF=carboxyfluorescein marker; IS=328 bp internal standard.
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Part Number |
Description |
| 477477 |
eCAP™ DNA Capillary - 100 µm ID, 65 cm total length (quantity 1). |
| 477628 |
eCAP™ ssDNA 1000 Gel Kit (pack of 3) |
| 477414 |
eCAP™ dsDNA 1000 Test Mix |
| 241524 |
eCAP™ Orange G Reference Marker |
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| Fluorescence Intercalator |
Part Number |
Description |
| 477409 |
eCAP™ EnhanCE Dye 0.5 mL |
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| eCAP™ ssDNA 100-R Kit, Part Number 477480 |
| For analysis of single-stranded DNA with linearity from 10 to 100 bases. Includes two coated capillaries, replaceable gel, and standards to perform at least 100 runs. UV detection is required to analyze unlabeled and standard oligonucleotides. Urea denaturant is included separately, enabling a naturing replaceable gel, if desired.
This kit is not compatible with a PDA detector.
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Analysis of 22-mer oligonucleotide
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Analysis of 22-mer oligonucleotide; 300 V/cm and 30 C. |
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Separation of synthetic 17-mer oligonucleotide
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Separation of synthetic 17-mer oligonucleotide with the trityl group attached to the 5' end. Insert shows high-speed separation of the same oligonucleotide accomplished using reverse injection from the short side of the capillary.
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Part Number |
Description |
| 477477 |
eCAP™ DNA Capillary - 100 µm ID, 65 cm total length (quantity 1). |
| 477621 |
ssDNA 100-R Gel Kit |
| 338481 |
eCAP™ ssDNA 100 Buffer Kit - Includes Tris borate buffer (2), Urea, 7 M (2). |
| 477626 |
PD(A) KIT, 40-60 |
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* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffmann-LaRoche, Ltd.
For Research Use Only; not for use in diagnostic procedures.
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